RESUMO
Here, we aim to improve the overall sustainability of aquaponic basil (Ocimum basilicum L.)-sturgeon (Acipenser baerii) integrated recirculating systems. We implement new AI methods for operational management together with innovative solutions for plant growth bed, consisting of Rapana venosa shells (R), considered wastes in the food processing industry. To this end, the ARIMA-supervised learning method was used to develop solutions for forecasting the growth of both fish and plant biomass, while multi-linear regression (MLR), generalized additive models (GAM), and XGBoost were used for developing black-box virtual sensors for water quality. The efficiency of the new R substrate was evaluated and compared to the consecrated light expended clay aggregate-LECA aquaponics substrate (H). Considering two different technological scenarios (A-high feed input, B-low feed input, respectively), nutrient reduction rates, plant biomass growth performance and additionally plant quality are analysed. The resulting prediction models reveal a good accuracy, with the best metrics for predicting N-NO3 concentration in technological water. Furthermore, PCA analysis reveals a high correlation between water dissolved oxygen and pH. The use of innovative R growth substrate assured better basil growth performance. Indeed, this was in terms of both average fresh weight per basil plant, with 22.59% more at AR compared to AH, 16.45% more at BR compared to BH, respectively, as well as for average leaf area (LA) with 8.36% more at AR compared to AH, 9.49% more at BR compared to BH. However, the use of R substrate revealed a lower N-NH4 and N-NO3 reduction rate in technological water, compared to H-based variants (19.58% at AR and 18.95% at BR, compared to 20.75% at AH and 26.53% at BH for N-NH4; 2.02% at AR and 4.1% at BR, compared to 3.16% at AH and 5.24% at BH for N-NO3). The concentration of Ca, K, Mg and NO3 in the basil leaf area registered the following relationship between the experimental variants: AR > AH > BR > BH. In the root area however, the NO3 were higher in H variants with low feed input. The total phenolic and flavonoid contents in basil roots and aerial parts and the antioxidant activity of the methanolic extracts of experimental variants revealed that the highest total phenolic and flavonoid contents were found in the BH variant (0.348% and 0.169%, respectively in the roots, 0.512% and 0.019%, respectively in the aerial parts), while the methanolic extract obtained from the roots of the same variant showed the most potent antioxidant activity (89.15%). The results revealed that an analytical framework based on supervised learning can be successfully employed in various technological scenarios to optimize operational management in an aquaponic basil (Ocimum basilicum L.)-sturgeon (Acipenser baerii) integrated recirculating systems. Also, the R substrate represents a suitable alternative for replacing conventional aquaponic grow beds. This is because it offers better plant growth performance and plant quality, together with a comparable nitrogen compound reduction rate. Future studies should investigate the long-term efficiency of innovative R aquaponic growth bed. Thus, focusing on the application of the developed prediction and forecasting models developed here, on a wider range of technological scenarios.
RESUMO
Metals are considered to be one of the most hazardous substances due to their potential for accumulation, magnification, persistence, and wide distribution in water, sediments, and aquatic organisms. Demersal fish species, such as turbot (Psetta maxima maeotica), are accepted by the scientific communities as suitable bioindicators of heavy metal pollution in the aquatic environment. The present study uses a machine learning approach, which is based on multiple linear and non-linear models, in order to effectively estimate the concentrations of heavy metals in both turbot muscle and liver tissues. For multiple linear regression (MLR) models, the stepwise method was used, while non-linear models were developed by applying random forest (RF) algorithm. The models were based on data that were provided from scientific literature, attributed to 11 heavy metals (As, Ca, Cd, Cu, Fe, K, Mg, Mn, Na, Ni, Zn) from both muscle and liver tissues of turbot exemplars. Significant MLR models were recorded for Ca, Fe, Mg, and Na in muscle tissue and K, Cu, Zn, and Na in turbot liver tissue. The non-linear tree-based RF prediction models (over 70% prediction accuracy) were identified for As, Cd, Cu, K, Mg, and Zn in muscle tissue and As, Ca, Cd, Mg, and Fe in turbot liver tissue. Both machine learning MLR and non-linear tree-based RF prediction models were identified to be suitable for predicting the heavy metal concentration from both turbot muscle and liver tissues. The models can be used for improving the knowledge and economic efficiency of linked heavy metals food safety and environment pollution studies.
